Applications
Sequence Capture/Targeted Region
Overview
Employ long range PCR products or DNA fragments captured with NimbleGen's Sequence Capture arrays to sequence as many as eight 1 MB captured regions with 30x oversampling on a single GS FLX Instrument run. Enrich and sequence all human exons stored in the CCDS database with NimbleGen's Sequence Capture arrays. Benefit from long reads for haplotyping of 200-300bp and straightforward identification of insertions and deletions from single to tens of base pairs, without bias against variation in GC content.
Roche Nimblegen Sequence Capture enables fast and accurate enrichment of thousands of selected genomic regions, either contiguous or dispersed (for example, segments of chromosomes genes, or exons) using a single microarray hybridization-based enrichment process.
Publications
- Albert et al. Direct selection of human genomic loci by microarray hybridization. Nature Methods 4: 903-5. November 2007.
- Yeager et al. Comprehensive resequence analysis of a 136 kb region of human chromosome 8q24 associated with prostate and colon cancers. Human Genetics. ePub August 14 2008.
Click here to see all Sequence Capture/Targeted Region publications.
Data Analysis tools:
GS Mapper Software
How It Works
Figure 1. NimbleGen Sequence Capture in combination with the Genome Sequencer FLX System. Genetic material is fragmented and targeted sequences are captured by hybridization to the sequence capture microarray. Captured DNA fragments are eluted from the array and sequenced on the GS FLX System.
For more information on Nimblegen Capture arrays visit http://www.nimblegen.com/products/seqcap/index.html.