Applications
Sequence Capture/Targeted Resequencing
Overview
Targeted resequencing allows scientists to capture or target only the regions of interest of the human genome and then use high-throughput sequencing to assess the genetic variation within the captured DNA fragments. NimbleGen Sequence Capture Arrays provide an optimal solution when paired with the GS FLX System and Titanium Series Reagents for targeted resequencing. The NimbleGen GS FLX Titanium Optimized Sequence Capture 385K Arrays can capture up to 5Mb (385K) genomic regions as enriched DNA fragments which can then be sequenced to the researchers desired depth and % base coverage using the GS FLX Titanium series reagents. In addition, the NimbleGen Sequence Capture 2.1M Human Exome arrays are capable of capturing the human exome, which consists of ~180,000 human coding exons and ~550 miRNA exons (from the CCDS database, build April 30th, 2008), on a single array. When combined with the GS FLX Titanium technology, the system offers long reads for haplotyping and straightforward identification of insertions and deletions, without GC bias.
Learn more about the NimbleGen Titanium Optimized Sequence Capture 385K Arrays
Learn more about the NimbleGen Sequence Capture 2.1 Human Exome Array
Publications
- D'Ascenzo et al. Mutation discovery in the mouse using genetically guided array capture and resequencing. Mammalian Genome. July 2009.
- Albert et al. Direct selection of human genomic loci by microarray hybridization. Nature Methods 4: 903-5. November 2007.
Click here to see all Sequence Capture/Targeted Region publications.
Data Analysis tools:
GS Mapper Software
How It Works
Figure 1: In the Sequence Capture workflow, 1) the genomic DNA sample is fragmented; 2) linkers are ligated to the fragments, and an optional amplification is performed; 3) the linkered DNA library is hybridized to a NimbleGen Sequence Capture array to allow specific binding of target regions to the array; 4) the unbound fragments are washed away, 5) target fragments are eluted off the array; 6) the eluted target fragment pool is amplified; 7) the enriched, amplified pool is verified by qPCR to measure fold enrichment; 8) the enriched sample is ready for high-throughput sequencing with the Genome Sequencer FLX System.
For more information on NimbleGen Sequence Capture arrays, visit www.nimblegen.com