Applications
Small RNA
Overview
Small RNAs, including microRNAs, endogenous siRNAs and Piwi-interacting RNAs, are a diverse collection of molecules with several important biological functions. The Genome Sequencer FLX System has the ability to rapidly sequence tens to hundreds of thousands of individual small RNA molecules for rapid identification and quantification in a sample, without cloning into bacteria. Discover novel small RNAs, explore small RNA biology, or characterize differential expression profiles by sequencing and comparing small RNA transcriptomes. 454 Sequencing system's highly accurate, long reads ensure that all classes of small RNAs are captured.
Application Note:
Publications
- Parameswaran et al. A pyrosequencing-tailored nucleotide barcode design unveils opportunities for large-scale sample multiplexing. Nucleic Acids Research 35: e130. 2007.
- Watanabe et al. Endogenous siRNAs from naturally formed dsRNAs regulate transcripts in mouse oocytes. Nature 453:539-43. May 22, 2008.
Click here to see all Small RNA publications.
How it Works:
Two methods are used to prepare small RNAs for sequencing on the Genome Sequencer FLX System:
- Ligation of the 44-mer standard A and B adaptors to small RNA after conversion into cDNA molecules
- Incorporation of A and B adaptor sequences during cDNA generation using Fusion Primers, as described for Amplicon Sequencing
Figure 1: Sequencing of small non-coding (snc) RNA via two different methods.