Applications
Somatic Variation Detection
Overview
The Genome Sequencer FLX system's long reads and ability to sequence from single DNA molecules enables straightforward identification of low frequency somatic mutations. Effectively detect the spectrum of variations in specific disease-associated regions or genes by sequencing PCR products, cDNA libraries, or DNA fragments captured with NimbleGen's Sequence Capture array.
Application Note:
Publications
- Thomas et al. Sensitive mutation detection in heterogeneous cancer specimens by massively parallel picoliter reactor sequencing. Nature Medicine 12: 852-5. July 2006.
- Wheeler et al. The complete genome of an individual by massively parallel DNA sequencing. Nature 452: 872-876. April 17, 2008.
- Yeager et al. Comprehensive resequence analysis of a 136 kb region of human chromosome 8q24 associated with prostate and colon cancers. Human Genetics. ePub August 14 2008.
Click here to see all Somatic Variation Detection publications.
Data Analysis tools:
GS Amplicon Variant Analyzer Software