Applications
Transcriptome Sequencing
Overview
The Genome Sequencer FLX System is a powerful platform for transcriptome sequencing, enabling a full range of applications including: genome annotation, identification of novel transcripts, splice variant detection, expression analysis (including allele-specific expression and the ability to distinguish paralogous genes), assembly of full-length genes, and discovery of SNPs or other variations such as indels. Obtain an unbiased transcriptome survey by sequencing full length cDNA libraries or expression tags with as few as one run of shotgun reads on the GS FLX System with GS FLX Titanium series reagents.
Application Note:
Publications
- Sugarbaker et al. Transcriptome sequencing of malignant pleural mesothelioma tumors. PNAS 105: 3521-3526. March 4, 2008.
- Salehi-Ashtiani et al. Isoform discovery by targeted cloning, 'deep-well' pooling and parallel sequencing. Nature Methods 5: 597-600. July 2008.
- Vera et al. Rapid transcriptome characterization for a non-model organism using 454 pyrosequencing. Molecular Ecology 17: 1636-47. April 2008.
Click here to see all Transcriptome Sequencing publications.
Data Analysis Tools:
GS De novo Assembler Software