Fields of Biology
Evolution & Ecology
Overview
The power of high throughput DNA sequencing opens new doors in the pursuit of the molecular basis of evolutionary and ecological relationships. Traditional barriers such as limited availability of precious DNA, complexity of an environmental sample, and economical and technical constraints have restricted genomic level research in the field of evolution and ecology. The Genome Sequencer FLX, with its long, highly accurate reads, is the ideal tool for genomic analysis of non-traditional species, living and extinct, along with their habitats and evolutionary relationships.
Applications
Ancient DNA
Obtain unprecedented insight into an organism's evolutionary history on a molecular level. The GS FLX System's long reads ensure the accurate assembly of whole or partial genomes of extinct species, and enable comparative genomics with living species.
Metagenomics
Characterize the molecular signatures of environmental habitats and gain insight into their ecology though metagenome or metatranscriptome sequencing. The GS FLX System's long reads and comprehensive results ensure fast and accurate identification of diverse members within microbial communities.
Whole genome sequencing - Eukaryote & Prokaryote Whole genome
Discover the evolutionary profile of an organism within its environment by sequencing its complete genome. High throughput shot-gun sequencing with the 454 Sequencing system and included data analysis tools enable rapid assembly of de novo sequences, in as little as a single run.
Transcriptome sequencing
Analyze the transcripts of expressed genes between unique species to better understand the evolution from genes to behavior. Obtain the complete transcriptome of a non-model organism by sequencing full-length cDNA libraries or expression tags.
Small RNA sequencing
Reveal details about the evolution of small RNAs by sequencing and comparing small RNA transcriptomes. The GS FLX System's comprehensive results ensure that all classes of small RNAs are captured.
Publications
- Green et al. A complete Neanderthal mitochondrial genome sequence determined by high-throughput sequencing. Cell 134: 416-426. August 8, 2008.
- Dinsdale et al. Functional metagenomic profiling of nine biomes. Nature 452: 629-32. April 3, 2008.
- Toth et al. Wasp Gene Expression Supports an Evolutionary Link Between Maternal Behavior and Eusociality. Science 318: 441-4. October 19, 2007.