Products & Solutions
GS Amplicon Variant Analyzer Software
- Automatically compute the alignment of reads from amplicon-based samples (e.g. ultra-deep amplicon sequencing) against a reference sequence
- Quickly identify variants and their respective frequencies in large, mixed-sample pools
- Screen against known variants (with reference) and discover unknown variants
- Perform haplotyping/phasing - identify linked variants over the full amplicon length
- Detect low-frequency (<1%) variants
- Graphically view alignments, position differences and % frequency with color coding
- Flexible project construction: separate samples/results based on MID tags, directly import assets into project, use filtered/modified input data files
- The GS Amplicon Variant Analyzer Software also enables:
- Resequencing: Analyze disease-associated regions, Discover SNPs, insertions, and deletions on a population level, Detect rare somatic mutations, Perform viral subtyping
- Epigenetics: Analyze DNA methylation patterns
- Metagenomics and genetic diversity: Sequence 16S RNA
- Data outputs: ace.file (alignment of the reads against a reference sequence), png.file (graphical file format, tab delimited text file)
Figure 1: GS Amplicon Variant Analyzer Software. Example of the Consensus Alignment screen displaying sequenced reads compared with a reference sequence. In this example, a 15 bp deletion is shown. The frequency of the variant and the number of reads are displayed in the upper part of the figure. The lower portion of the figure shows the multiple alignment of the individual reads used to generate the variation plot. The reference sequence is shown at the top of the alignment, and the deletion is centered in the window. The vertical yellow bar in the center of the deletion highlights a selected base.