Products & Solutions
Multiplexing
Physical Separation of Samples
Tailor sample number and throughput to your specific needs.
- Gaskets physically divide the PicoTiterPlate (PTP) device into smaller regions (1, 2, 4, 8, and 16 regions).
- Simultaneous sequence multiple samples on a single PTP while preserving the unique identity of each sample during sequencing and data analysis.
Multiplex Identifiers (MIDs)
Now available for the GS FLX Titanium Series—Contact your local sales representative for more information.
Molecular barcodes, called Multiplex Identifiers (MIDs), are used to specifically tag unique samples in a GS FLX Titanium sequencing run. A kit with a basic set of 12 MIDs is available for use with the GS FLX Titanium Rapid Library preparation procedure. An additional set of 14-141 customer-generated MIDs are available for GS FLX Titanium amplicon sequencing. The MID-tagged samples can be amplified by emPCR and sequenced together to streamline the workflow. Each MID is recognized by the GS FLX data analysis software after the sequencing run, providing high confidence in assigning an individual sequencing read to the correct sample.
MIDs expand multiplexing options beyond physical separation of samples
- Reduce the cost per sample by pooling many samples in a single run.
- Simplify sample preparation by eliminating the need for individual amplification reactions.
- Expand experimental design options by multiplexing samples.
- Obtain more reads per sample by reducing the needs for gaskets, making more PTP wells available for sequencing.
- Reduce the risk of contamination by tagging each DNA molecule with a sample-specific tag.
Figure: Ligation MID workflow. Using the Ligation MIDs, 12 individual samples can be pooled for emPCR and sequencing. Downstream of sequencing, the GS FLX software automatically identifies and assigns each read by the sample-specific MID sequence.