Products & Solutions
Multiplexing
Physical Separation of Samples
Tailor sample number and throughput to your specific needs.
- Gaskets physically divide the PicoTiterPlate (PTP) device into smaller regions (1, 2, 4, 8, and 16 regions).
- Simultaneous sequence multiple samples on a single PTP while preserving the unique identity of each sample during sequencing and data analysis.
| Gasket Format (regions) | Reads/Region (x103) |
|---|---|
| 2 | 450-650 |
| 4 | 160-250 |
| 8 | 80-120 |
| 16 | 25-40 |
Table 1: Gasket format options and reads per region for GS FLX Titanium series kits.
Multiplex Identifiers (MIDs)
Now available for the GS FLX Titanium Series—Contact your local sales representative for more information.
Uniquely tag up to twelve samples with new Ligation Multiplex Identifiers (MIDs). Twelve MID adaptors are available, each containing a unique 10-nucleotide sequence. During library preparation, sample-specific MID adaptors molecules are blunt-end ligated onto each DNA fragment in a sample. The MID-tagged samples can be pooled for simultaneous amplification and sequencing in a single region. Used in conjunction with the available gaskets, up to 192 samples can be sequenced per run. Each MID is recognized by the GS FLX analysis software, allowing for automated grouping and analysis of MID-containing reads.
MIDs expand multiplexing options beyond physical separation of samples
- Reduce the cost per sample by pooling many samples in a single run.
- Simplify sample preparation by eliminating the need for individual amplification reactions.
- Expand experimental design options by multiplexing samples.
- Obtain more reads per sample by reducing the needs for gaskets, making more PTP wells available for sequencing.
- Reduce the risk of contamination by tagging each DNA molecule with a sample-specific tag.
Figure: Ligation MID workflow. Using the Ligation MIDs, 12 individual samples can be pooled for emPCR and sequencing. Downstream of sequencing, the GS FLX software automatically identifies and assigns each read by the sample-specific MID sequence.