Products & Solutions
Roche NimbleGen Sequence Capture
NimbleGen Sequence Capture arrays enable you to quickly produce targeted, sequencing-ready samples in your lab for use with the Genome Sequencer FLX System and GS FLX Titanium Kits.
- Target Any Region of Interest: Capture up to 30Mb total regions, such as the whole human exome, on a single 2.1M array and up to 5Mb on a single 385K array with high coverage and specificity.
- Generate Data with Confidence: Ensure the highest level of specificity and sensitivity with an empirically optimized and validated capture design algorithm.
- Detect Important Variants: The unique 400 bp long reads from the GS FLX Titanium series enable easy detection of indels, improve coverage in repetitive regions, and provide haplotype information.
- Easily Analyze Data: Dedicated GS Reference Mapper software reports variant locations, amino acid changes in coding regions, and known SNP information. In addition, the GS Mapper software generates capture performance metrics, such as percentage reads in target regions.
Product Offerings
NimbleGen 454 Optimized Sequence Capture 385K Arrays
Quickly Identify Causative Mutations in Disease-Associated Regions
The NimbleGen 454 Optimized Sequence Capture 385K arrays have been optimized to work seamlessly with the GS FLX System. Together, these technologies combine to offer a powerful solution for large-scale targeted resequencing studies to significantly reduce time, labor and cost while improving data quality.
NimbleGen SeqCap EZ Exome Library and 454 GS FLX Titanium Series
Genetic Discovery Made Easy
NimbleGen Sequence Capture arrays enable you to produce targeted, sequencing-read samples in your lab. Target the exome using a single tube of stable DNA oligos and a seamless sequencing workflow. With 2.1 million empirically optimized DNA probes, SeqCap EZ Exome Library delivers a high level of performance and substantial savings on sequencing.
How it Works
Figure 1: Sequence Capture protocols.
- Genomic DNA: An array or SeqCap EZ oligo pool is made against target regions in the genome.
- Library Preparation: Standard shot-gun sequencing library is made from genomic DNA.
- Hybridization: The sequencing library is hybridized to the SeqCap EZ oligo pool or to the Sequence Capture array.
Steps 4 and 5 are different for each protocol:
SeqCap EZ, biotinylated DNA oligos in solution
- Bead Capture: Streptavidin beads are used to pull down the complex of capture oligos and genomic DNA fragments.
- Washing: Unbound fragments are removed by washing.
Sequence Capture, capture probes synthesized on array:
- Washing: Unbound fragments are removed by washing.
- Target Fragment Elution: The enriched fragment pool is eluted and recovered from the array.
- Amplification: Enriched fragment pool is amplified by PCR.
- Enrichment QC: The success of enrichment is measured by qPCR at control loci.
- Sequencing-Ready DNA: The end product is a sequencing library enriched for target regions, ready for high throughput sequencing.
For more information on NimbleGen Sequence Capture arrays, visit www.nimblegen.com/seqcap