Products & Solutions
Roche NimbleGen Sequence Capture
NimbleGen Sequence Capture arrays enable you to quickly produce targeted, sequencing-ready samples in your lab for use with the Genome Sequencer FLX System and GS FLX Titanium Kits.
- Target Any Region of Interest: Capture up to 30Mb total regions, such as the whole human exome, on a single 2.1M array and up to 5Mb on a single 385K array with high coverage and specificity.
- Generate Data with Confidence: Ensure the highest level of specificity and sensitivity with an empirically optimized and validated capture design algorithm.
- Detect Important Variants: The unique 400 bp long reads from the GS FLX Titanium series enable easy detection of indels, improve coverage in repetitive regions, and provide haplotype information.
- Easily Analyze Data: Dedicated GS Reference Mapper software reports variant locations, amino acid changes in coding regions, and known SNP information. In addition, the GS Mapper software generates capture performance metrics, such as percentage reads in target regions.
Product Offerings
NEW! NimbleGen Titanium Optimized Sequence Capture 385K Arrays
Quickly Identify Causative Mutations in Disease-Associated Regions
The NimbleGen GS FLX Titanium Optimized Sequence Capture 385K arrays have been optimized to work seamlessly with the GS FLX System. Together, these technologies combine to offer a powerful solution for large-scale targeted resequencing studies to significantly reduce time, labor and cost while improving data quality.
NimbleGen Sequence Capture 2.1 Human Exome Array
Revolutionizing Genetic Disease Research
NimbleGen Sequence Capture arrays enable you to produce targeted, sequencing-read samples in your lab. Utilizing high density, long-oligonucleotide Nimblegen 2.1 M arrays and an optimized algorithm, the entire human exome, including ~180,000 coding exons and 551 miRNA exons, can be captured on a single array. A Titanium optimized protocols is available.
How it Works
Figure 1: In the Sequence Capture workflow, 1) the genomic DNA sample is fragmented; 2) linkers are ligated to the fragments, and an optional amplification is performed; 3) the linkered DNA library is hybridized to a NimbleGen Sequence Capture array to allow specific binding of target regions to the array; 4) the unbound fragments are washed away, 5) target fragments are eluted off the array; 6) the eluted target fragment pool is amplified; 7) the enriched, amplified pool is verified by qPCR to measure fold enrichment; 8) the enriched sample is ready for high-throughput sequencing with the Genome Sequencer FLX System.
For more information on NimbleGen Sequence Capture arrays, visit www.nimblegen.com/seqcap