Products & Solutions
Sample Prep Options
Long Single Reads
A GS FLX Titanium series sequencing run generates more than 1,000,000 single reads with an average read length of 400 bases. Highly versatile, these single long reads can be applied to almost every application, and open the full spectrum of genomic variation to analysis. Whether the targets are SNPs, insertions or deletions, novel genes or genomic regions, long single reads enable you to investigate the entire genome.
Long-Tag Paired End Reads
The GS FLX Standard series supports 3K Long-Tag Paired End Reads. Sequence 100 bases from each end of a 3,000 base span on a single sequence read (Figure). Co-assemble GS FLX Titanium shotgun reads with 3K Long-Tag Paired Ends reads from Standard series runs. Stay tuned for GS FLX Titanium series long-tag paired ends with 3K-20K spans!
- Significantly improve the de novo assemblies of complex genomes.
- Identify structural variations at high resolution, including insertions, deletions, inversions and copy number variations.
- Straightforward approach yields faster and more accurate results than other next-generation sequencing methods.
Roche NimbleGen Sequence Capture
Roche NimbleGen Sequence Capture enables fast and accurate enrichment of thousands of selected genomic regions, either contiguous or dispersed (for example, segments of chromosomes, genes, or exons) using a single microarray hybridization- based enrichment process. Use this powerful technology in conjunction with the GS FLX System to quickly and economically sequence the targeted genomic regions for downstream analysis.
Figure 1. NimbleGen Sequence Capture in combination with the Genome Sequencer FLX System. Genetic material is fragmented and targeted sequences are captured by hybridization to the sequence capture microarray. Captured DNA fragments are eluted from the array and sequenced on the GS FLX System.
For more information on NimbleGen Sequence Capture arrays, click here.
Amplicon Sequencing
Currently limit to Standard Series
Amplicon sequencing can be used for a variety of applications with the Genome Sequencer FLX System:
- Discovery of rare somatic mutations in complex samples (e.g. cancerous tumors- mixed with germline DNA) based on ultra-deep sequencing.
- Sequencing collections of exons from populations of individuals to identify diversity.
- Sequencing collections of human exons from populations of individuals for the identification of rare alleles associated with disease.
- Analysis of viral quasispecies present within infected populations in the context of epidemiological studies.
- Evolutionary biology in populations.
Amplicon sequencing Targeted DNA regions prepared via PCR can be entered directly into the emPCR process, bypassing the library preparation stage. The DNA-sample preparation for Amplicon Sequencing with the GS FLX System consists of a simple PCR amplification reaction with special Fusion Primers. The Fusion Primer consists of a 20-25 bp target-specific sequence (3' end) and a 19 bp fixed sequence (Primer A or Primer B on the 5' end).
Order your 454 FusionPrimers directly from Integrated DNA Technologies. Design, synthesis and purify primers for with the 454 Genome Sequencer FLX System.
emPCR Formats (Titanium Series)
New Large Volume emPCR kit simplifies library preparation workflow compared to the standard emPCR protocol, reducing the number of emulsions for a full run from 32 to two (Small Volume emPCR remains available for experiments running multiple small samples). High-performance Taq polymerase is now included in the GS FLX Titanium series emPCR kits, reducing the cost-per-run.