Products & Solutions
System Features
GS FLX Titanium Series:
New Reagents for Today's GS FLX Instrument
The GS FLX Titanium series reagents run on the Genome Sequencer FLX Instrument, a system based on 454's sequencing-by-synthesis technology. The GS FLX Titanium series improves on the current system with upgraded reagents, consumables, and software.
- Innovative redesign of the PicoTiterPlate device, including a higher density of wells and metallic coating using smaller DNA capture beads, enables improvements in both the number and length of reads while facilitating exceptional accuracy
- Improved sequencing chemistry provides longer reads and more robust performance.
- Updated software is optimized for ultra-high throughput applications, easing the assembly and mapping of larger, more complex genomes.
- GS FLX Version 2.0 software is fully compatible with data output from previous versions, allowing reads to be co-assembled and mapped.
| Throughput | 400-600 million high-quality, filter-passed bases per run* 1 billion bases per day |
| Run Time | 10 hours |
| Read Length | Modal length = 500 bases, Average length = 400 bases |
| Accuracy | Q20 read length of 400 bases (99% at 400 bases and higher for prior bases) |
| Reads per run | >1 million high-quality reads |
| Data | Trace data accepted by NCBI since 2005 |
| Computing Requirements | Cluster recommended (Roche GS FLX Titanium Cluster available) |
| Robustness | No complex optics or lasers; reagents have long shelf life |
Table 1: GS FLX Titanium series performance
* Typical result, average read length and number of reads depend on specific sample characteristics.
GS FLX Standard Series
| Throughput | 100 million high-quality filter-passed bases per run, 200 million bases per day* |
| Run Time | 7.5 hours |
| Read Length & Accuracy | 250 bases with >99.5% single-read accuracy* |
| Reads per run | 400,000 high-quality reads* |
| Data | Trace data accepted by NCBI since 2005 |
| Computing Requirements | Single Linux server |
| Robustness | No complex optics or lasers, long-life reagents |
*Typical result, average read length and number of reads depend on specific sample characteristics.
GS FLX Instrument
The Genome Sequencer instrument (Figure 1) is the centerpiece of the Genome Sequencer FLX System. The fluidics subsystem ensures accurate reagent dispensing. It consists of a reagents cassette holding the reagent containers, a sipper manifold, pumps, valves, and debubblers. The fluidics subsystem flows the sequencing reagents across the wells of the PicoTiterPlate device, and moves the spent reagents from the PicoTiterPlate device to the waste receptacle.
The optics subsystem consists of a CCD camera and a camera controller. The camera captures the light emitted in the wells of the PicoTiterPlate device during each step of the sequencing cycle, and sends the digital images to the computer subsystem for processing.
Figure 1: Genome Sequencer FLX Instrument
| Dimensions: upper assembly | 74.3 cm W × 69.8 cm D × 36.1 cm H (29.25 in W × 27.5 in D × 14.2 in H) including monitor 82.5 cm (32.5 in) H |
| Dimensions: lower assembly | 75.2 cm W × 90.8 cm D × 92.7 cm H (29.62 in W × 35.75 in D× 36.5 in H) |
| Weight | 242 kg (532 lbs) |
| Power supply | 85 - 132 Vac 50/60Hz 173 - 264 Vac 50/60Hz |
| Noise level | <65 dB(A) |
| Electromagnetic emission | Class A Important: This is a Class A device. In residential areas, this device may cause radio interference. The user should take the necessary precautions, if appropriate. |
| Electromagnetic immunity | Important: In industrial areas, this device may be influenced by radio interference. The user should take the necessary precautions, if appropriate. |