Genome Sequencing; DNA, PCR, & MicroRNA Analysis; De Novo Assembly

How much input DNA is required?: 5ug of double stranded DNA.
What quality of DNA is required?: Provide the DNA at a concentration > 300ng/ul and A260/280 ratio of approximately 1.8. Providing an image of DNA sample(s) run on a 2% agarose gel is requested to assess quality of incoming DNA.
What coverage is needed?: De novo sequencing requires 20X average genome coverage. Resequencing and mapping project require 15X average coverage.
How long do projects take?: Length of a project depends on number of samples, genome size and data analysis requirements. Typical bacterial projects are normally completed within a month of receipt of sample.
Has anyone done a project like mine before?: Browse through a selected list of projects completed at 454 in the Services section. Fill out a Request for Service form to talk to one of our account managers for more detail.
How should samples be shipped?: Click here for sample submission guidelines (.pdf)
What should the DNA be resuspended in?: H2O or 1XTE is preferred.
How much input DNA is required? And volume?: 5ug of double stranded DNA, no less than 10 ul.
What If my DNA is 500 bp long or less, how much do you really need?: At that point there is no nebulization. 2 ug is enough, no less than 10 ul in volume.

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